Ort Vector Machine Classifier enables correct classification of 94 samples at a sensitivity of 0.889 and a specificity of 1 (one chordoma sample was not correctly classified). The receiver operating characteristics (ROC) derived from the Bayesian Compound Covariate MK-8931 Predictor provides an area under the curve AUC of 0.952. Although theparametric p-values of several single gene qPCR ct values were below p,0.05, the classification success is very impressive. Generation of a novel classifier from the entire set of 48 qPCR amplicons applying the feature selection criteria “Genes with univariate misclassification rate below 0.2” for class prediction elucidates a classifier of 23 genes enabling perfect classification of the entire set of study samples (AUC = 1) by the Compound Covariate Predictor, the 1-Nearest Neighbor and the Bayesian Compound Covariate Predictor. Correct classification of 94 was obtained by using the Diagonal Discriminant, the Nearest Centroid, and the Support Vector Machines analyses. The 3Nearest Neighbor classification success was 88 (Table S3). For reducing the classifier to a lower number of genes feature selection by “univariate p-value ,0.05 and 2 fold -change between classes” was applied and class prediction was performed again on the entire set of all the 48 amplicons used for qPCR. Thereby a classifier for distinction between peripheral blood and chordoma was generated. This classifer consisted of qPCR-ct methylation measures of RASSF1, KL, C3, HIC1, RARB, TACSTD2, XIST, and FMR1 (Table 4). That classifier enabled perfect classification of the set of study samples (AUC = 1) by the 1-Nearest Neighbor method. Correct classification of 94 was obtained by using the Compound Covariate Predictor and the Support Vector Machines. The classification success was 88 achieved by the Diagonal Discriminant Analyses, the Nearest Centroid, and analyses and the 3-Nearest Neighbors classifier. The Bayesian Compound Covariate Predictor allowed also perfect classification. Two samles, however, could not be classified (indicated as “NA” in Table S4).DNA Methylation and SNP Analyses in ChordomaTable 3. Composition of the classifier derived from class prediction (Sorted by t -value): HIC1 presented by two different probes on the CpG360 array is present twice in two lines.Parametric p-value 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 O R O K E H A I D J S M Q L B F N C G P 1.9e-06 7.87e-05 0.0002284 0.0002639 0.0005252 0.0020097 0.0034824 0.0043484 0.0055942 0.0057031 0.0063306 0.0065378 0.006866 0.0084843 0.Mirin web 0097382 0.0096666 0.0085768 0.0044802 0.0038254 0.t-value 27.254 25.254 24.726 24.655 24.323 23.684 23.424 23.318 23.199 23.189 23.14 23.124 23.101 23 22.934 2.937 2.995 3.304 3.379 3.CV support 100 100 100 100 100 100 100 100 72 56 56 33 50 33 28 28 22 100 100Geom mean of intensities in blood 117.83 122.83 1680.69 204.18 99.87 240.07 1786.2 9598.38 69.16 132.37 3185.91 255.63 1157.46 186.9 3585.36 98.26 3744.86 274.47 577.96 298.Geom mean of intensities in chordoma 5002.77 389.47 45724.96 2114.22 2091.96 3056.36 6777.17 22361.92 181.48 592.55 5503.58 4661.49 2159.2 3110.51 33560.67 62.79 979.1 114.11 182.72 122.Fold-change 0.024 0.32 0.037 0.097 0.048 0.079 0.26 0.43 0.38 0.22 0.58 0.055 0.54 0.06 0.11 1.56 3.82 2.41 3.16 2.Gene symbol HIC1 CTCFL HIC1 ACTB RASSF1 CDX1 GBP2 IRF4 DLEC1 COL21A1 GNAS KL C3 SRGN S100A9 HSD17B4 BAZ1A STAT1 NEUROG1 JUPThe letters (A ) can be found in Figure 2, where SNP data are combined w.Ort Vector Machine Classifier enables correct classification of 94 samples at a sensitivity of 0.889 and a specificity of 1 (one chordoma sample was not correctly classified). The receiver operating characteristics (ROC) derived from the Bayesian Compound Covariate Predictor provides an area under the curve AUC of 0.952. Although theparametric p-values of several single gene qPCR ct values were below p,0.05, the classification success is very impressive. Generation of a novel classifier from the entire set of 48 qPCR amplicons applying the feature selection criteria “Genes with univariate misclassification rate below 0.2” for class prediction elucidates a classifier of 23 genes enabling perfect classification of the entire set of study samples (AUC = 1) by the Compound Covariate Predictor, the 1-Nearest Neighbor and the Bayesian Compound Covariate Predictor. Correct classification of 94 was obtained by using the Diagonal Discriminant, the Nearest Centroid, and the Support Vector Machines analyses. The 3Nearest Neighbor classification success was 88 (Table S3). For reducing the classifier to a lower number of genes feature selection by “univariate p-value ,0.05 and 2 fold -change between classes” was applied and class prediction was performed again on the entire set of all the 48 amplicons used for qPCR. Thereby a classifier for distinction between peripheral blood and chordoma was generated. This classifer consisted of qPCR-ct methylation measures of RASSF1, KL, C3, HIC1, RARB, TACSTD2, XIST, and FMR1 (Table 4). That classifier enabled perfect classification of the set of study samples (AUC = 1) by the 1-Nearest Neighbor method. Correct classification of 94 was obtained by using the Compound Covariate Predictor and the Support Vector Machines. The classification success was 88 achieved by the Diagonal Discriminant Analyses, the Nearest Centroid, and analyses and the 3-Nearest Neighbors classifier. The Bayesian Compound Covariate Predictor allowed also perfect classification. Two samles, however, could not be classified (indicated as “NA” in Table S4).DNA Methylation and SNP Analyses in ChordomaTable 3. Composition of the classifier derived from class prediction (Sorted by t -value): HIC1 presented by two different probes on the CpG360 array is present twice in two lines.Parametric p-value 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 O R O K E H A I D J S M Q L B F N C G P 1.9e-06 7.87e-05 0.0002284 0.0002639 0.0005252 0.0020097 0.0034824 0.0043484 0.0055942 0.0057031 0.0063306 0.0065378 0.006866 0.0084843 0.0097382 0.0096666 0.0085768 0.0044802 0.0038254 0.t-value 27.254 25.254 24.726 24.655 24.323 23.684 23.424 23.318 23.199 23.189 23.14 23.124 23.101 23 22.934 2.937 2.995 3.304 3.379 3.CV support 100 100 100 100 100 100 100 100 72 56 56 33 50 33 28 28 22 100 100Geom mean of intensities in blood 117.83 122.83 1680.69 204.18 99.87 240.07 1786.2 9598.38 69.16 132.37 3185.91 255.63 1157.46 186.9 3585.36 98.26 3744.86 274.47 577.96 298.Geom mean of intensities in chordoma 5002.77 389.47 45724.96 2114.22 2091.96 3056.36 6777.17 22361.92 181.48 592.55 5503.58 4661.49 2159.2 3110.51 33560.67 62.79 979.1 114.11 182.72 122.Fold-change 0.024 0.32 0.037 0.097 0.048 0.079 0.26 0.43 0.38 0.22 0.58 0.055 0.54 0.06 0.11 1.56 3.82 2.41 3.16 2.Gene symbol HIC1 CTCFL HIC1 ACTB RASSF1 CDX1 GBP2 IRF4 DLEC1 COL21A1 GNAS KL C3 SRGN S100A9 HSD17B4 BAZ1A STAT1 NEUROG1 JUPThe letters (A ) can be found in Figure 2, where SNP data are combined w.
