Oxidative stress and produced higher levels of reactive oxygen species (ROS
Oxidative stress and produced higher levels of reactive oxygen species (ROS) under stress in accordance with recent reports. The susceptibility to oxidative stress-induced cell death in AMD RPE-iPSC-RPE and Skin-iPSC-RPE was consistent with inability of the AMD RPE-iPSC-RPE and Skin-iPSCRPE to increase SOD2 expression under oxidative stress. Phenotypic analysis revealed disintegrated mitochondria, accumulation of autophagosomes and lipid droplets in AMD RPE-iPSC-RPE and AMD Skin-iPSC-RPE. Mitochondrial activity was significantly lower in AMD RPE-iPSC-RPE and AMD Skin-iPSC-RPE compared to normal cells and glycogen concentration was significantly increased in the diseased cells. Furthermore, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1), a regulator of mitochondrial biogenesis and function was repressed, and lower expression levels of NAD-dependent deacetylase sirtuin1 (SIRT1) were found in AMD RPE-iPSC-RPE and AMD Skin-iPSC-RPE as compared to normal RPE-iPSC-RPE. Conclusions: Our studies suggest SIRT1/PGC-1 as underlying pathways contributing to AMD pathophysiology, and open new avenues for development of targeted drugs for treatment of this devastating neurodegenerative disease of the visual system. Keywords: AMD, RPE, Oxidative stress, Mitochondria, Cell viability, ROS, PGC-1, SIRT*Correspondence: [email protected] 1 Department of Ophthalmology, Georgetown University Medical Center, 3900 Reservoir Road NW, Medical-Dental Building, Room NE203, Washington, DC 20057, USA Full list of author information is available at the end of the article?The Author(s) 2016. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Golestaneh et al. J Transl Med (2016) 14:Page 2 ofBackground Age related macular degeneration (AMD) is a major cause of blindness in the developed countries, primarily affects the retinal pigment epithelium (RPE) resulting in subsequent degeneration of the photoreceptors [1?]. AMD is a multifactorial disease with a complex interaction between environmental, metabolic and hereditary factors [5]. Clinically, AMD is presented in two forms, non-exudative and exudative. The non-exudative or dry form of AMD is diagnosed by polymorphic deposits, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27532042 called drusen, that accumulate underneath the RPE and can result in overlying macular atrophy and pigmentation [6]. The exudative, or wet form of AMD is characterized by choroidal neovascularization leading to hemorrhage, retinal fluid, and eventual disciform scar formation [7]. However, an individual could present both forms at different stage of life, or initially develop dry form that would transform into wet form at later stage of the disease. To date, there is no effective treatment for dry AMD, yet millions of patients continue to lose their vision worldwide. Because of its complex etiology, understanding the molecular mechanisms of AMD has been ONO-4059MedChemExpress Tirabrutinib challenging by lack of the appropriate in vitro model that could sufficiently recapitulate the charac.
