Rmoxic ventilation of rabbit lungs in the presence (+SOD) and the absence of SOD (-SOD). After 3 h, PMA was injected into the pulmonary artery, resulting in a concentration of 1 in the recirculating buffer fluid. The increase in ESR Pan-RAS-IN-1 biological activity signal intensity was linear before and after addition of PMA. The insert shows the PMA effect with higher time resolution. (B) Changes in the increase rate of the ESR signal intensity ( ) by comparison of values prior to and after addition of PMA to isolated rabbit lungs. In the +SOD group, SOD was present throughout the experiments. In two separate sets of experiments, a fiber oxygenator was used instead of the lung for oxygenation of the recirculating buffer fluid (“fiber oxygenator”). The fibre oxygenator experiments were performed either in the absence (“fiber oxygenator”) or in the presence of 1 FeCl2 (“fiber oxygenator + FeCl2”) in the buffer fluid. * significant difference between the +SOD and the -SOD groupPage 8 of(page number not for citation purposes)Respiratory Research 2005, 6:http://respiratory-research.com/content/6/1/change in increase rate of signal intensity ( )A)300 250 200 150control control +SOD apocynin rotenoneB)change in increase rate of signal intensity ( )500 450 400 350 300 250 200 150 100 WT WT + SOD gp91phox-/gp91phox-/+ SOD* **Figure 5 signal of the NADPH oxidase and lungs after addition of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28499442 phorbol-12-myristate-13-acetate (PMA) during deletion ventilation Effectsintensity in isolated perfused mitochondrial inhibitors, as well as of phagocytic NADPH oxidase genenormoxic on the ESR Effects of the NADPH oxidase and mitochondrial inhibitors, as well as of phagocytic NADPH oxidase gene deletion on the ESR signal intensity in isolated perfused lungs after addition of phorbol-12-myristate-13-acetate (PMA) during normoxic ventilation. (A) Effect of the NADPH oxidase inhibitor apocynin (500 ) and the inhibitor of mitochondrial complex I, rotenone (350 nM) on the increase rate in ESR signal intensity after addition of PMA. Each inhibitor was added to the buffer fluid 30 min before addition of PMA. In the +SOD group, SOD was present throughout the experiments. * significant difference as compared to control. (B) Comparison of the increase rate in ESR signal intensity after addition of PMA in wildtype (WT) and gp91phox-deficient (gp91phox-/-) mice. Lungs were perfused for 120 min prior to PMA addition (10 ) either in the presence or absence of 150 U/ml SOD. * significant difference as compared to WT. Data are given as changes in the increase rate of the ESR signal intensity after PMA addition, as compared to the values before PMA addition (set as 100 ). Data are from n = 4? experiments for each group.Page 9 of(page number not for citation purposes)Respiratory Research 2005, 6:http://respiratory-research.com/content/6/1/Table 1: Baseline pulmonary artery pressure (PAP) and phorbol-12-myristate-13-acetate (PMA)-induced changes in PAP after 3 hours of normoxic ventilation. Pulmonary artery pressure values (PAP) are given for time points directly before and 6 min after addition of PMA to the buffer fluid. In addition, the increase in PAP per minute (PAP/min) after PMA addition is indicated. Data are shown for experiments in the absence (-SOD) and the presence of 150 U/ml superoxide dismutase (+SOD). The PMA was added after 3 h of normoxic ventilation. Values of the +SOD and -SOD group correspond to experiments in Fig. 4. In the apocynin group this agent was present in the perfusate at a c.
