Cosins A and B (regioisomers arisingFrontiers in Plant Science www.frontiersin.orgNovember Volume ArticleSisTerraza et al.Coumarins in FeDeficient Arabidopsis PlantsTABLE MSMS information for a few of the compounds secreted and accumulated by Arabidopsis thaliana roots in response to Fe deficiency mz ratios of your fragment ions and their relative intensity.Compound # Annotation Parent ion mz ..Ion form ESIMSn mz (Relative intensity, in )Coniferylaldehyde hexoside ,dihydroxymethoxycoumarin hexoside (fraxetin hexoside) hydroxy,dimethoxycoumarin hexoside (isofraxidin hexoside)[MH] [MH]MS , , , , , , MS MS , , , , , , MS MS , , , , , , , , , , , , , , , , , , , , , , , , , , MS MS , , , , , , , , , , , , , MS MS , , MS , , , , MS , , , , , , .[MH]Sinapyl aldehyde hexoside.[MH] Ferulic acid Coniferyl aldehyde Sinapyl aldehyde…[MH] [MH] [MH]Numbers in italics (Compound #) refer for the labels applied for every compound in Table .All information had been taken from the HPLCESIMSMS(ion trap) evaluation.The big ion from the MS and MS spectra can also be indicated in bold.in the fusion of fraxetin and the monolignol hydroxyconiferyl alcohol, Cheng and Chen, , Figure C), have a formula identical to that of compound (C H O).The structural variations amongst these coumarinolignans corresponding towards the monolignol moiety (Figure B) are identical to those found among the elemental formulae of (i) a methoxy group differentiates coniferyl from sinapyl alcohols as well as the elemental formula of from that of ; (ii) a hydroxyl group differentiates hydroxyconiferyl from coniferyl alcohols plus the elemental formula of from that of ; and (iii) a methyl group differentiates hydroxyconiferyl and sinapyl alcohols and also the formula of from these of .To confirm the identification of as cleomiscosins, we isolated coumarinolignans from C.viscosa seeds.The seed isolate was analyzed by both HPLCUVVISESIMS(TOF) and HPLCESIMS(ion trap) working with Elution program and optimistic ESI ionization.The HPLCESIMS(TOF) chromatogram for mz . corresponding for the cleomiscosins C and D [MH] ions, (E)-LHF-535 Epigenetics showed only two peaks, at .and .min, matching together with the RTs of and (Figure C; Table).Similarly, the HPLCESIMS(TOF) chromatogram for mz . corresponding to the cleomiscosins A and B [MH] ions, showed only two peaks, at .and .min, matching together with the RTs of (Figure C; Table).Peaks were assigned to cleomiscosin isomers as outlined by the elution order reported in the literature (Chattopadhyay et al Kaur et al).These annotations were confirmed by the full match among the MS spectra with the cleomiscosins D, C, B, and a, and those of compounds , , and , respectively (Figure C).Compound eluted at shorter times than the cleomiscosins (.vs..min), as expected in the structural variations in between hydroxycleomiscosin A and B and cleomiscosins (see above).Moreover, compound shares elemental formula and the presence of a fraxetin moietywith hydroxycleomiscosins A and B, and its MS spectrum showed a loss of Da from the [MH] ion (Figure B; Supplementary Table S), which was previously reported for hydroxycleomiscosin A (Cheng and Chen,) but doesn’t happen in cleomiscosins.As a result, was putatively annotated as hydroxycleomiscosin A andor B (Table ).Coumarin and Coumarinolignan Concentrations in Root ExtractsQuantification of phenolic compounds was carried out using the PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21541725 [MH] and [MhexosideH] signals inside the HPLCESIMS(TOF).Coumarin.
