Nuscript; readily available in PMC 2019 February 15.Li et al.Pageco-transformed into tobacco leaf epidermal cells together with a construct expressing SLR1 tagged together with the C-terminus of YFP. Scale bar, 60 m. The photographs of BiFC assays represent among the 3 experiments performed independently with similar final results (panels b and c).Europe PMC Funders Busulfan-D8 Cell Cycle/DNA Damage Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure six. SLR1 inhibits OsGRF4-OsGIF1 self-promotion of OsGRF4 mRNA and OsGRF4 protein abundance.a, OsGFR4 mRNA abundance, plant genotypes as indicated. Leukotriene E4 Autophagy Abundance shown relative to that in NJ6 (=1). Data shown as imply s.e.m. (n = three). Diverse letters denote important variations (P 0.05, Duncan’s multiple variety test). b, The effects of GA and PAC on OsGRF4 mRNA abundance in 2-week-old NJ6 plants. Abundance shown relative to that in water remedy control (=1). Data shown as mean s.e.m. (n = three). Distinctive letters denote important differences (P 0.05, Duncan’s numerous variety test). c, ChIP-PCR OsGRF4mediated enrichment (relative to input) of GCGG-containing OsGRF4 promoter fragments (marked with ). Information shown as mean s.e.m. (n = 3). Unique letters denote important differences (P 0.05, Duncan’s several range test). d, OsGRF4-activated promotion of transcription in the OsGRF4 gene promoter-luciferase reporter construct is enhanced byNature. Author manuscript; readily available in PMC 2019 February 15.Li et al.PageOsGIF1 and inhibited by SLR1. Abundance of LUCREN shown relative to that in empty vector handle (=1). Information shown as imply s.e.m. (n = three). Diverse letters denote important differences (P 0.05, Duncan’s a number of variety test). e, OsGRF4 abundance (as detected by an anti-OsGRF4 antibody), plant genotypes as indicated. HSP90 serves as loading handle. The images of western blots represent among the list of 3 experiments performed independently with similar final results.Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure 7. The OsGRF4-SLR1 antagonism regulates carbon assimilation and plant growth.a, b, Relative shoot abundances of C-fixation gene mRNAs. Abundances of transcripts of genes regulating photosynthesis (a), sucrose metabolism and transportphloem loading (b) in NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 plants. Information shown as imply s.e.m. (n = 3). Abundances in NJ6 and NJ6-sd1-OsGRF4ngr2 expressed relative to NJ6-sd1 (= 1). Distinctive letters denote considerable variations (P 0.05, Duncan’s multiple variety test). c, ChIP-PCR assays. Diagram depicts the OsPsbS1, OsTPS1 and OsSWEET11 promoters and regions applied for ChIP-PCR, and GCGG-containing promoter fragment (marked with ) enrichment (relative to input). Information shown as imply s.e.m. (n = 3). Various letters denote substantial differences (P 0.05, Duncan’s several range test). d, Transactivation assays. The LUCREN activity obtained from a co-transfection with an empty effector construct andNature. Author manuscript; obtainable in PMC 2019 February 15.Li et al.Pageindicated reporter constructs was set to become one. Information shown as imply s.e.m. (n = 9). Diverse letters denote significant variations (P 0.05, Duncan’s several range test). e, Immunoblot detection of OsLhca1, OsLhca3, OsLhca4, OsLhcb2, OsPsaD and OsPsaE utilizing antibodies as shown in genotypes as indicated. HSP90 serves as loading manage. The photos of western blots represent one of several 3 experiments performed independently with related outcomes. f-i, Comparisons.
