)(d)Figure 6. Adjustments in Kyoto Encyclopedia ofof Genes and Genomes (KEGG
)(d)Figure 6. Adjustments in Kyoto Encyclopedia ofof Genes and Genomes (KEGG) enrichment pathway fruits of two late-ripening Figure 6. Modifications in Kyoto Encyclopedia Genes and Genomes (KEGG) enrichment pathway in in fruits of two late-ripensweet oranges, LW LW (a,b) and XC (c,d) just after foliar spraying of very easily extractable glomalin-related soil protein(EE-GRSP). ing sweet oranges, (a,b) and XC (c,d) soon after foliar spraying of simply extractable glomalin-related soil protein (EE-GRSP). PHA-543613 site Abbreviation: LW_EG, the LW wide variety sprayed with EE-GRSP; LW_Non_EG, the LW wide variety not sprayed with EE-GRSP; Abbreviation: LW_EG, the LW selection sprayed with EE-GRSP; LW_Non_EG, the LW range not sprayed with EE-GRSP; XC_EG, the XC variety sprayed with EE-GRSP; XC_Non_EG, the XC variety not sprayed with EE-GRSP. XC_EG, the XC wide variety sprayed with EE-GRSP; XC_Non_EG, the XC variety not sprayed with EE-GRSP.Within the XC range, the KEGG enrichment pathway was divided into human diseases, organismal systems, metabolism, and environmental information and facts processing, of whichAgriculture 2021, 11,9 ofTable 2. Gene species of starch and sucrose metabolism.Sweet Oranges Up-Regulated Genes Cs3g23560 (-amylase), Cs1g18220 (-fructofuranosidase), Cs8g13800 (glycoside hydrolase loved ones 3), orange1.1t00611 (ADP-glucose pyrophosphorylase signature 2), Cs5g33760 (glycosyl hydrolase family 9), Cs9g18290 (trehalose 6-phosphate phosphatase), Cs3g26050 (hexokinase loved ones signature), Cs5g01280 (granule-bound starch synthase 1), UCB-5307 Data Sheet Cs2g12180 (glycosyl hydrolase family 9), and Cs4g11970 (glycosyl hydrolase family 1 signature) Up-Regulated Gene Number Down-Regulated Genes Down-Regulated Gene NumberLWCs7g01380 (glycoside hydrolase family 1), Cs4g06900 (sucrose synthase), Cs5g19060 (sucrose-phosphate synthase four), Cs2g07740 (glycosyl hydrolase household 1 signature), and Cs1g01330 (trehalose 6-phosphate phosphatase)XCCs5g19060 and Cs7g08390 (-trehalose-phosphate synthase [UDP-forming] 9)Cs9g06360 (ADP-glucose pyrophosphorylase signature 1), Cs7g11210 (glycosyl hydrolase household 1), Cs9g14600 (trehalose-phosphate phosphatase A), Cs1g18220, orange1.1t03668 (sucrose-phosphate synthase three), Cs4g02730 (-trehalose-phosphate synthase [UDP-forming] 11), Cs5g09200 (trehalose-phosphate phosphatase F), Cs3g18450 (trehalose-phosphate phosphatase C), orange1.1t00483 (trehalose 6-phosphate phosphatase), Cs2g12180, and orange1.1t03470 (plant -amylase signature)3.8. Transcription Element Loved ones Analysis We also analyzed the transcription element loved ones. A total of 2946 transcription things have been involved, belonging to 30 transcription issue households, which represented the families of far more than 100 transcription factors for instance C3H, C2H2, bHLH, NAC, far1, MYB connected, Agriculture 2021, 11, x FOR PEER Evaluation ERF, MYB, bZIP, and ARF (Figure 7). These responses indicated that a big variety of 10 of 13 transcription factors have been triggered by the foliar application of EE-GRSP.Figure 7. Figure 7. Changes in transcription aspect families in two sweet under the condition of foliar spray of very easily Alterations in transcription aspect households in two sweet orange varieties orange varieties below the situation extractable glomalin-related effortlessly extractable glomalin-related soil protein (EE-GRSP). of foliar spray of soil protein (EE-GRSP).four. Discussion Our preceding research established the improvement in various fruit high-quality parameters of late-ripening sweet oranges (particularly LW variety) in response to foliar spray of EE-GRSP. Howe.
