F our experiments were also performed in COS-7 and 293 cells (of monkey and human embryonic kidney origin, respectively), and yielded results equivalent to HUVECs (see Fig. 9), Also, results constant with our findings have been reported inside a number of other cell varieties, like human airway [21] and rat smooth muscle cells [31].NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptAcknowledgmentsWe are thankful to Dr. James Darnell, Jr. for kindly offering us with mStat3 wt and mutant expression vectors. Funding This study was supported by Plan Project Grant HL-65608 to LCB in the National Heart, Lung and Blood Institute, and from start-up funds in the Cardiovascular Institute, University of Pittsburgh, to SNM.Biochim Biophys Acta. Author manuscript; offered in PMC 2013 May 01.Mattagajasingh et al.Web page
bioengineeringReviewBioengineering of Extracellular Vesicles: Exosome-Based Next-Generation Therapeutic Tactic in CancerPriyanka Saha, Suchisnigdha Datta, Sukanya Ghosh, Anurima Samanta, Paramita Ghosh and Dona Sinha Department of Receptor Biology and Tumor Metastasis, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, India; [email protected] (P.S.); [email protected] (S.D.); [email protected] (S.G.); [email protected] (A.S.); [email protected] (P.G.) Correspondence: [email protected] or [email protected]; Tel.: ++91-33-2476-5101 (ext. 327); Fax: +91-33-2475-Abstract: Extracellular nano BRD4 Modulator drug Vesicles and exosomes hold compelling proof in intercellular communication. Exosomal intracellular signal transduction is mediated by the transfer of cargo proteins, lipids, micro (mi)RNAs, long noncoding (lnc)RNAs, tiny interfering (si)RNAs, DNA, and also other functional molecules that play a pivotal part in regulating tumor development and metastasis. However, emerging analysis trends indicate that exosomes could be used as a promising tool in anticancer treatment. This overview options a majority from the bioengineering applications of fabricated exosomal cargoes. Additionally, it encompasses how the manipulation and delivery of specific cargoes–noncoding RNAs (ncRNAs), recombinant proteins, immune-modulators, chemotherapeutic drugs, and also other smaller molecules–may serve as a precise therapeutic strategy in cancer management.Citation: Saha, P.; Datta, S.; Ghosh, S.; Samanta, A.; Ghosh, P.; Sinha, D. Bioengineering of Extracellular Vesicles: Exosome-Based Next-Generation Therapeutic Approach in Cancer. Bioengineering 2021, eight, 139. https://doi.org/ 10.3390/bioengineering8100139 Academic Editor: Prakash Gangadaran Received: four September 2021 Accepted: five October 2021 Published: 10 OctoberKeywords: exosome; bioengineering; immunotherapy; exosomal cargo delivery; recombinant proteins; ncRNA; chemotherapy1. Introduction Specialized nanoparticles, exosomes, have gained considerable focus from researchers and clinicians by virtue of their intercellular communication and effective drug delivery house [1]. Exosomes are very advantageous for therapeutic purposes resulting from their higher stability, targetability, less immunogenicity, prolonged COX Inhibitor manufacturer half-life, and capacity to cross the blood rain barrier (BBB) [2]. Exosomes might be modified with several molecules, chemotherapeutic drugs, functional proteins, and genetic components, which may possibly emerge as a prospective next-generation anti-cancer approach. Exosomes may possibly invade physiological barriers, which have been generally imp.
