Tochem Cell Biol. 1997;108(1):67?5. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, de Angelis P, Claussen OP. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod Oxf Engl. 1999;14(4):1039?9. Evenson DP. Sperm chromatin structure assay, SCSAR. Methods Mol Biol. 2013;927:147?4. Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. Nutlin (3a) cost Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells. Exp Cell Res. 1993;207(1):202?. Klaude M, Eriksson S, Nygren J, Ahnstr G. The comet assay: mechanisms and technical considerations. Mutat Res. 1996;363(2):89?6. Fern dez JL, Muriel M, Rivero MT, Goyanes V, Vazquez R, Alvarez JG. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl. 2003;24(1):59?6. Fern dez JL, V quez-Gund F, Delgado A, Goyanes VJ, Ramiro-D z J, de la Torre J, Gos vez J. DNA breakage detection-FISH (DBD-FISH) in human spermatozoa: technical variants evidence different structural features. Mutat Res. 2000;453(1):77?2. Ohno M, Sakumi K, Fukumura R, Furuichi M, Iwasaki Y, Hokama M, Ikemura T, Tsuzuki T, Gondo Y, Nakabeppu Y. 8-oxoguanine causes spontaneous de novo germline mutations in mice. Sci Rep. 2014;4:4689. WHO laboratory manual for the examination and processing of human semen. WHO. http://apps.who.int/iris/bitstream/10665/44261/1/9789241547789_eng.pdf. Giwercman A, Lindstedt L, Larsson M, Bungum M, Spano M, Levine RJ, Rylander L. Sperm chromatin structure assay as an independent predictor of fertility in vivo: a case ontrol study. Int J Androl. 2010;33(1):221?. Evenson DP, Darzynkiewicz Z, Melamed MR. Relation of mammalian sperm chromatin heterogeneity to fertility. Science. 1980;210(4474):1131?. Nygren KG, Sullivan E, Zegers-Hochschild F, Mansour R, Ishihara O, Adamson GD, de Mouzon J. International Committee for Monitoring AssistedChamproux et al. Basic and Clinical Andrology (2016) 26:Page 22 of210. 211.212.213.214.Reproductive Technology (ICMART) world report: assisted reproductive technology 2003. Fertil Steril. 2011;95(7):2209?2. Palermo GD, Neri QV, Takeuchi T, Rosenwaks Z. ICSI: where we have been and where we are going. Semin Reprod Med. 2009;27(2):191?01. Hourcade JD, P ez-Crespo M, Fern dez-Gonz ez R, Pintado B, Guti rezAd A. Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage. Reprod Biol Endocrinol. 2010;8:9. Osman A, Alsomait H, Seshadri S, El-Toukhy T, Khalaf Y. The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta- analysis. Reprod Biomed Online. 2015;30(2):120?. Kumar SB. Improvement in Sperm DNA Quality Following Simple Life Style Intervention: A Study in Fathers of Children with Non-Familial Sporadic Heritable Retinoblastoma. J Clin Case Rep. 2015;5:509. doi:10.4172/2165-7920. 1000509. Gharagozloo P, Guti rez-Ad A, Champroux A, Noblanc A, Kocer A, Calle A, P ez-Cerezales S, Pericuesta E, Polhemus PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26162776 A, Moazamian A, Drevet JR, Aitken RJ. A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models. Hum Reprod. 2016;31(2):252?2.Submit your next manuscript to BioMed Central and we will help you at every step:?We accept pre-submission inquiries ?Our selector tool helps you to find.