iersin.orgAugust 2021 | Volume 12 | ArticleTorres-Silva et al.De novo Transcriptome of M. glaucescens Shoot OrganogenesisFIGURE six | Networks of differentially expressed genes. The networks had been created using GO categories in BiNGO for M. glaucescens explants prior to (handle) and immediately after (treated) shoot organogenesis induction. Bubble size and color indicate the frequency of the GO term and also the P-value, respectively.Frontiers in Plant Science | frontiersin.orgAugust 2021 | Volume 12 | ArticleTorres-Silva et al.De novo Transcriptome of M. glaucescens Shoot OrganogenesisTABLE 3 | Transcription aspect families containing downregulated and upregulated 5-HT3 Receptor Antagonist MedChemExpress unigenes following M. glaucescens shoot organogenesis induction. Transcription factor household Domain No. of unigenes Downregulated Upregulated AP2 AP2 zf-Dof DUF260 B3 and auxin response B3 zf-LSD1 G2-like GRAS Homeobox Homeobox zf-CCCH NAM bZIP_1 WRKY Myb_DNA-binding Myb_DNA-binding HLH EIN3 HSF_DNA-bind zf-C2H2 Trihelix GATA 2 1 0 five 1 0 two two 1 0 4 0 3 1 4 0 1 0 4 0 1 three 0 0 0 0 0 1 three 1 0 0 0 1 0 1 1 five 1 7 0 4 11 5 2 two 3 3 1 5 three 17 5 2 10 four 4 three two 7 8 five 2 2 1 1 0 1 0 1 1 1 1 1 0 0 0ERF family protein AP2 loved ones protein Dof family protein LBD family members protein ARF family protein B3 loved ones protein LSD family members protein G2-like household protein GRAS family members protein HB-other household protein TALE loved ones protein C3H loved ones protein NAC household protein bZIP family protein WRKY family members protein MYB household protein MYB_related loved ones protein bHLH household protein EIL family protein HSF family protein C2H2 loved ones protein Trihelix loved ones protein GATA family members proteinHD-ZIP household protein HomeoboxE2F/DP household protein E2F_TDP CAMTA family protein CG-1 NF-YC household protein NF-YA family members protein NF-X1 loved ones protein ZF-HD loved ones protein SBP loved ones protein YABBY loved ones protein FAR1 family protein MIKC_MADS family protein GRF family members protein S1Fa-like household protein TCP family protein DBB family protein NF-YC CBFB_NFYA zf-NF-X1 ZF-HD_dimer SBP YABBY FAR1 SRF-TF and K-box WRC and QLQ S1FA TCP zf-B_boxCO-like loved ones protein zf-B_box and CCTdivision and cell wall formation in the Trk Purity & Documentation course of regeneration (Bao et al., 2009). In addition, modifications in cell fate can take place as a result of a new balance involving euchromatin and heterochromatin following dynamic alterations in chromatin structure (Zhao et al., 2008), indicated here by the presenceof histone-encoding genes among the upregulated transcripts (Figure five). Transcription aspect households and unigenes have been additional abundant among the upregulated than downregulated unigenes (Table three). This finding indicates that many metabolic pathways are activated for the duration of shoot organogenesis induction. The households of transcription factors identified to become upregulated within this study (i.e., NF-Y, MYB, ERF, E2F, LBD, and NAC) are akin to those identified in the course of plant organogenesis, additional confirming the function of those regulators in vegetative regeneration (Cheong et al., 2002; Bao et al., 2009; Cervantes-P ez et al., 2018; Nadiya et al., 2018; Pal et al., 2018; Xi et al., 2019). In the course of the in vitro culture preceding shoot organogenesis induction, the metabolism of M. glaucescens was characterized by fewer upregulated than downregulated genes, corroborating the low development price standard of cacti cultures (Lema-Rumiska and n Kulus, 2014; P ez-Molphe-Balch et al., 2015). Downregulated genes had been largely involved in key metabolism. They incorporated transcripts connected to photosynthesis (15) and antenna proteins (5) identified in KEGG
